Contemporary Neurology
Epstein-Barr virus (EBV)-transformed human B lymphocytes and the … clinical and basic topics of human neurology, neurosciences and related fields. Editor …
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We used reverse tran-scriptase polymerase chain reaction (RT-PCR) to examine four samplesof highly purified human B lymphocytes, isolated from peripheralblood, before and after EBV transformation for the presence of trk bmRNA transcript. Our results showed that in all samples, trk b mRNAwas undetectable in resting B lymphocytes but was expressed followingEBV transformation. Materials and Methods Cell preparation and culture One unit of heparinized venous blood was obtained from each of fourhealthy adult volunteers. From each sample, 10 mL were diluted 1:1 inRPMI 1640 medium (GIBCO BRL, Gaithersburg, MD) and centrifugedover Ficoll-Paque (Pharmacia Biotech, Piscataway, NJ) gradient. Perma-nent EBV transformation of unfractionated mononuclear leukocyteswas
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